Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| BH3 interacting domain death agonist | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
Bid |
Protein |
BH3-interacting domain death agonist |
Uniprot ID |
P70444 |
Function |
Induces caspases and apoptosis. Counters the protective effect of Bcl-2. The major proteolytic product p15 BID allows the release of cytochrome c. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
Apoptic death agonist antibody|Apoptotic death agonist antibody|Apoptotic death agonist BID antibody|BH3 interacting domain death agonist antibody|BH3 interacting domain death agonist p11 antibody|BH3 interacting domain death agonist p13 antibody|BH3 interacting domain death agonist p15 antibody|BH3-interacting domain death agonist p11 antibody|BID antibody|BID isoform ES(1b) antibody|BID isoform L(2) antibody|BID isoform Si6 antibody|BID_HUMAN antibody|Desmocollin type 4 antibody|FP497 antibody|HGNC:1050 antibody|Human BID coding sequence antibody|MGC15319 antibody|MGC42355 antibody|p11 BID antibody|p13 BID antibody|p15 BID antibody|p22 BID antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bid antibody, ASA-B0229, IHC(P)
IHC(P): Rat Spleen Tissue
IHC(P): Rat Spleen Tissue
Anti-Bid antibody, ASA-B0229, Western blotting
All lanes: Anti Bid(ASA-B0229) at 0.5ug/ml
WB: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
All lanes: Anti Bid(ASA-B0229) at 0.5ug/ml
WB: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 22KD
Observed bind size: 22KD
