Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| integrin-binding sialoprotein |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
IHC-P, WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein(17-34aa FSMKNLHRRVKIEDSEEN), different from the related rat and mouse sequences by three amino acids. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
IBSP |
Protein
|
Bone sialoprotein 2 |
Uniprot ID
|
P21815 |
Function
|
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment. |
Tissue Specificity
|
|
Sub-cellular localization
|
Secreted. |
Sequence Similarities
|
|
Aliases
|
BNSP antibody|Bone sialoprotein 2 antibody|Bone sialoprotein 2 precursor antibody|Bone sialoprotein II antibody|bone sialoprotein II; bone sialoprotein antibody|BSP antibody|BSP II antibody|BSPII antibody|Cell binding sialoprotein antibody|Cell-binding sialoprotein antibody|IBSP antibody|Integrin binding sialoprotein antibody|Integrin binding sialoprotein(bone sialoprotein II) antibody|integrin binding sialoprotein(bone sialoprotein, bone sialoprotein II) antibody|Integrin-binding sialoprotein antibody|SIAL_HUMAN antibody|SPII antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human, Rat Mouse |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human Mouse |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Bone Sialoprotein antibody, ASA-B0239, IHC(P)
IHC(P): Human Osteosarcoma Tissue
