Description
Data Sheet
| Applications | Western blot with anti-Sm positive patient sera. |
| Coating concentration | 0.4-0.8 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups. |
| Description | Bovine Small Nuclear Ribonucleoprotein Polypeptides purified from bovine tissues by proprietary protein-chemical techniques. |
| Expression host | Bovine tissues. |
| Formulation | SNRPD is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels). |
| Inactivation | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.718 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of TGF-b 3 as a Reference Standard. |
| Purity | Greater than 60% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
