Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| carbonic anhydrase I | Polyclonal | IgG | Rabbit | Human, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human CA1 recombinant protein (Position: D9-F261). Human CA1 shares 78.5% and 81% amino acid (aa) sequence identity with mouse and rat CA1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CA1 |
Protein |
Carbonic anhydrase 1 |
Uniprot ID |
P00915 |
Function |
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
CA 1 antibody|CA I antibody|CA-I antibody|CA1 antibody|CAB antibody| CAH1_ HUMAN antibody|CAI antibody|CAN antibody|Car 1 antibody|Car1 antibody| Carbonate dehydratase I antibody| Carbonic anhydrase 1 antibody|Carbonic anhydrase B antibody|Carbonic anhydrase I antibody| Carbonic dehydratase antibody|ECK0125 antibody|JW0122 antibody|yadF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CA1 antibody, ASA-B0258, Western blotting
All lanes: Anti CA1 (ASA-B0258) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 29KD
Observed bind size: 29KD
All lanes: Anti CA1 (ASA-B0258) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Predicted bind size: 29KD
Observed bind size: 29KD
Anti-CA1 antibody, ASA-B0258, IHC(P)
IHC(P): Human Liver Cancer Tissue
IHC(P): Human Liver Cancer Tissue
