Calbindin-D Antibody (monoclonal)

Calbindin-D Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0263 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
calbindin 1, 28kDa Monoclonal IgG Mouse Bovine, Human, Mouse, Rat IHC-P, WB Ascites

Immunogen
Bovine kidney calbindin-D

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CALB1

Protein

 Calbindin

Uniprot ID

 Human(P05937), Mouse(P12658), Rat(P07171)

Function

 Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3′,5′-cyclic nucleotide phosphodiesterase.

Tissue Specificity

Sub-cellular localization

Sequence Similarities

 Belongs to the calbindin family.

Aliases

 CALB|D-28K|CALBINDIN, 28-KD|Calbindin D28|Calbindin D|Vitamin D-dependent calcium-binding protein, avian-type

Application Details

Application Concentration* Species Validated Using**
Western blot 0.5-1μg/ml Human, bovine, mouse, rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 1-2μg/ml Human, bovine, mouse, rat AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Calbindin-D antibody (monoclonal), ASA-B0263, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate

Anti-Calbindin-D antibody (monoclonal), ASA-B0263, IHC(P)
IHC(P): Mouse Kidney Tissue
Anti-Calbindin-D antibody (monoclonal), ASA-B0263, IHC(P)
IHC(P): Rat Kidney Tissue

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