Caldesmon(smooth) Antibody (monoclonal)

Caldesmon(smooth) Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0266 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caldesmon 1 Monoclonal IgG Mouse Human, Mouse, Rabbit IHC-P, WB Ascites

Immunogen
Human uterus smooth muscle extract.

Properties

Form

 Liquid

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

Storage

 At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CALD1

Protein

 Caldesmon

Uniprot ID

 Human(Q05682), Mouse(Q8VCQ8), Rat(Q62736)

Function

 Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity).

Tissue Specificity

High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

Sub-cellular localization

 Cytoplasm, cytoskeleton . Cytoplasm, myofibril . Note: On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle).

Sequence Similarities

Aliases

 CDM|H-CAD|L-CAD

Application Details

Application Concentration* Species Validated Using**
Western blot 0.25-0.5μg/ml Human, mouse, rabbit AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Human, mouse, rabbit AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Caldesmon(smooth) antibody, ASA-B0266, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti-Caldesmon(smooth) antibody, ASA-B0266, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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