Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| calponin 2 |
Monoclonal |
IgG |
Mouse |
Chicken, Human, Turkey |
IHC-P, ICC, WB |
Ascites |
Immunogen
|
| Turkey gizzard calponin |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
CNN2 |
Protein
|
Calponin-2 |
Uniprot ID
|
Human(Q99439), Mouse(Q08093) |
Function
|
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. |
Tissue Specificity
|
Heart and smooth muscle. |
Sub-cellular localization
|
|
Sequence Similarities
|
Belongs to the calponin family. |
Aliases
|
Calponin H2, smooth muscle|Neutral calponin |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.25-0.5μg/ml |
Human, chicken, turkey |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
0.5-1μg/ml |
Human, chicken, turkey |
AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry |
Suitable |
Human, chicken, turkey |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
