Description
Data Sheet
| Description | Calsequestrin is the major calcium storage protein of the sarcoplasmic reticulum. Intraluminar Ca2+ binds to calsequestrin during diastole to prevent Ca2+ precipitation and to lower its free ionic concentration to facilitate efficient storage. During systole, Calsequestrin coordinately releases ~40-50 Ca2+ ions per molecule for each contraction-relaxation cycle by an uncertain mechanism. Calsequestrin has been shown to be of major importance in the regulation of cardiac excitation-contraction coupling. |
| Expression host | Dog Heart. |
| Formulation | The protein was lyophilized from a concentrated solution (1mg/ml) containing 10mM Tris-HCl and 1mM EGTA. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility | It is recommended to reconstitute the lyophilized CASQ2 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized CASQ2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CASQ2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Calsequestrin-2, Calsequestrin cardiac muscle isoform, CASQ2, CSQ. |
