Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| carbonic anhydrase I | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Carbonic Anhydrase I(51-68aa SYNPATAKEIINVGHSFH), different from the mouse sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CA1 |
Protein |
Carbonic anhydrase 1 |
Uniprot ID |
P00915 |
Function |
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
CA 1 antibody|CA I antibody|CA-I antibody|CA1 antibody|CAB antibody|CAH1_HUMAN antibody|CAI antibody|CAN antibody|Car 1 antibody|Car1 antibody|Carbonate dehydratase I antibody|Carbonic anhydrase 1 antibody|Carbonic anhydrase B antibody|Carbonic anhydrase I antibody|Carbonic dehydratase antibody|ECK0125 antibody|JW0122 antibody|yadF antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Carbonic Anhydrase I antibody, ASA-B0277, Western blotting
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: Rat Kidney Tissue Lysate
Lane 4: Rat Lung Tissue Lysate
