Description
Data Sheet
| Antibody Properties | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Description | Recombinant Human CBR3 fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated polypeptide chain containing 297 amino acids (1-277 a.a) and having a molecular mass of 33kDa. CBR3 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The CBR3 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Immunological function | This antibody is also available conjugated to FITC and biotin. For flow cytometry use 5-10ul/106 cells. |
| Protein Background | CBR3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. CBR3 is one of several monomeric NADPH-dependent oxidoreductases. Furthermore, CBR3 contains 3 exons spanning 11.2 kilobases and is strongly linked to another carbonyl reductase gene, the CBR1. It was suggested that CBR3 mediates 9-cis-retinoic acid-induced cytostatis and is a potential prognostic marker for oral malignancy.CBR3 is identified in the ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and the testis.Polymorphisms in CBR3 may give an explanation to interindividual and interethnic variability of doxorubicin pharmacokinetics and pharmacodynamics. |
| Purity | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Specificity | CD31 is expressed in high levels on endothelial cells and in lower levels on monocytes, granulocytes and platelets. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Synonyms | Carbonyl reductase [NADPH] 3, NADPH-dependent carbonyl reductase 3, CBR3, carbonyl reductase 3, hCBR3, SDR21C2. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
