Description
FOR RESEARCH USE ONLY
Data Sheet
| 10X Reaction Buffer | 200 mM HEPES, 50 mM MgCl2, 1 M NaCl, 1 mM EDTA, pH 6.5 |
| Application | Create double blunt end for recombination |
| Concentration | 1 µg/ µL, 6.26 µM |
| Description | Recombinant enzyme purified from E.coli. Cas9 Nuclease is an RNA-guided endonuclease that catalyzes site-specific cleavage of double stranded DNA. |
| Formulatin | 100μg in 100μl of 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. |
| Host/Source | An E. coli strain that carries recombinant Cas9 gene from Streptococcus pyogenes |
| Physical Appearence | Colorless solution. |
| Purity | Greater than 95.0% as determined by SDS-PAGE analysis. |
| Size | 50 µg, 313 pmol |
| Storage buffer | 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol. |
| Storage/ Stability | All components are stable for 1 year from the date of shipping when stored and handled properly. Store all components at -20°C. Avoid repeated freeze- thaw cycles of all components to retain maximum performance. |
