Description
Data Sheet
| Amino acid sequence | MSSSEEVSWI SWFCGLRGNE FFCEVDEDYI QDKFNLTGLN EQVPHYRQAL DMILDLEPDE ELEDNPNQSD LIEQAAEMLY GLIHARYILT NRGIAQMLEK YQQGDFGYCP RVYCENQPML PIGLSDIPGE AMVKLYCPKC MDVYTPKSSR HHHTDGAYFG TGFPHMLFMV HPEYRPKRPA NQFVPRLYGF KIHPMAYQLQ LQAASNFKSP VKTIR. |
| Description | Casein Kinase 2 beta Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 24.9 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | CSNK2B lyophilized from 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 1mM EDTA, protease inhibitor cocktail, pH 8.5. |
| Protein Background | The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes.Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility | Solubilize in distilled H2O or 50% glycerol. |
| Stability | Lyophilized CSNK2B although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CSNK2B should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Synonyms | Casein kinase II subunit beta, CK II beta, Phosvitin, G5a, CK2B, CK2N, CSK2B, MGC138222, MGC138224. |
