Caspase-3(P17) Antibody

Caspase-3(P17) Antibody

$330.00

In stock

SKU: ASA-B0295 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
caspase 3, apoptosis-related cysteine peptidase Polyclonal IgG Rabbit Mouse, Rat IHC-P, WB Immunogen affinity purified.

Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Caspase-3(P17)(67-81aa TDVDAANLRETFMGL), different from the related rat sequence by one amino acid.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CASP3

Protein

 Caspase-3

Uniprot ID

 P70677

Function

 Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a ‘216-Asp-

Tissue Specificity

Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.

Sub-cellular localization

 Cytoplasm.

Sequence Similarities

Aliases

 A830040C14Rik antibody|Apopain antibody|Apopain precursor antibody|CASP 3 antibody|CASP-3 antibody|CASP3 antibody|Casp3a antibody|Caspase 3 antibody|Caspase 3 apoptosis related cysteine protease antibody|Caspase 3 p17 subunit antibody|Caspase 3, apoptosis-related cysteine peptidase antibody|Caspase 3, apoptosis-related cysteine protease antibody|Caspase 3, apoptosis-related cysteine protease a antibody|Caspase-3 subunit p17 antibody|Caspase3 antibody|CC3 antibody|CPP 32 antibody|CPP-32 antibody|CPP32 antibody|CPP32B antibody|Cysteine protease CPP32 antibody|EC 3.4.22.56 antibody|Human cysteine protease CPP32 isoform alpha mRNA complete cds antibody|ICE3 antibody|LICE antibody|mldy antibody|OTTHUMP00000165052 antibody|OTTHUMP00000165053 antibody|OTTHUMP00000165054 antibody|PARP cleavage protease antibody|Procaspase3 antibody|Protein Yama antibody|SCA 1 antibody|SCA-1 antibody|SCA1 antibody|SREBP cleavage activity 1 antibody|Yama antibody|Yama protein antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Mouse, Rat AssaySolutio’s ECL kit
Immunohistochemistry(Paraffin-embedded Section) 0.5-1μg/ml Rat Mouse AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Caspase-3(P17) antibody, ASA-B0295, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Liver Tissue Lysate
Lane 3: Rat Thymus Tissue Lysate
Lane 4: Rat Spleen Tissue Lysate
Anti-Caspase-3(P17) antibody, ASA-B0295, IHC(P)
IHC(P): Rat Intestine Tissue Lysate

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