Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine(C-C motif) receptor 3 | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CCR3(1-20aa MTTSLDTVETFGTTSYYDDV). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCR3 |
Protein |
C-C chemokine receptor type 3 |
Uniprot ID |
P51677 |
Function |
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. |
Tissue Specificity |
In eosinophils as well as trace amounts in neutrophils and monocytes. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
Belongs to the G-protein coupled receptor 1 family. |
Aliases |
B Chemokine Receptor antibody|b-chemokine receptor antibody|C C chemokine receptor type 3 antibody|C C CKR 3 antibody|C-C chemokine receptor type 3 antibody|C-C CKR-3 antibody|CC Chemokine Receptor 3 antibody|CC chemokine receptor type 3 antibody|CC CKR 3 antibody|CC R3 antibody|CC-CKR-3 antibody|CCCKR3 antibody|CCR 3 antibody|CCR-3 antibody|CCR3 antibody|CCR3_HUMAN antibody|CD 193 antibody|CD193 antibody|CD193 antigen antibody|Chemokine(C C motif) receptor 3 antibody|chemokine(C-C motif) receptor 3 antibody|Chemokine(CC) Receptor 1 Like 2 antibody|Chemokine CC Motif Receptor 3 antibody|Chemokine receptor 3 antibody|CK R3 antibody|CKR 3 antibody|CKR3 antibody|CMKB R3 antibody|CMKBR 3 antibody|CMKBR1L2 antibody|CMKBR3 antibody|Eosinophil CC Chemokine Receptor 3 antibody|Eosinophil Eotaxin Receptor antibody|Eotaxin Receptor antibody|Macrophage inflammatory protein 1 alpha receptor like 2 antibody|Macrophage Inflammatory Protein1 Alpha Receptor Like 2 antibody|MGC102841 antibody|MIP1 Alpha RL2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CCR3 antibody, ASA-B0332, Western blotting
Lane 1: K562 Cell Lysate
Lane 2: RAJI Cell Lysate
Lane 1: K562 Cell Lysate
Lane 2: RAJI Cell Lysate
Anti-CCR3 antibody, ASA-B0332, IHC(P)
IHC(P): Human Tonsil Tissue
IHC(P): Human Tonsil Tissue
