Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine(C-C motif) receptor 4 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence in the middle region of mouse CCR4(346-360aa, SYTQSTVDHDFRDAL), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCR4 |
Protein |
C-C chemokine receptor type 4(C-C CKR-4/CC-CKR-4/CCR-4/CCR4) |
Uniprot ID |
P51680 |
Function |
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol- calcium second messenger system. Could play a role in lipopolysaccharide (LPS)-induced endotoxic shock. In the CNS, could mediate hippocampal-neuron survival. |
Tissue Specificity |
Expressed in the thymus, macrophages and T- and B-cells. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
C C chemokine receptor type 4 antibody|C C CKR 4 antibody|C-C chemokine receptor type 4 antibody|C-C CKR-4 antibody|CC CKR 4 antibody|CC-CKR-4 antibody|CCR 4 antibody|CCR-4 antibody|CCR4 antibody|CCR4_HUMAN antibody|CD194 antibody|Chemokine(CC motif) receptor 4 antibody|chemokine C C motif receptor 4 antibody|ChemR13 antibody|CKR4 antibody|CMKBR 4 antibody|CMKBR4 antibody|HGCN 14099 antibody|K5 5 antibody|K5-5 antibody|MGC88293 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CCR4 antibody, ASA-B0334, Western blotting
All lanes: Anti CCR4 (ASA-B0334) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti CCR4 (ASA-B0334) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Spleen Tissue Lysate at 50ug
Lane 3: HEPA Whole Cell Lysate at 40ug
Predicted bind size: 41KD
Observed bind size: 41KD
