Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human CD11a recombinant protein (Position: Q148-E523). Human CD11a shares 77% mino acid (aa) sequence identity with mouse CD11a. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
ITGAL |
Protein |
Integrin alpha-L |
Uniprot ID |
P20701 |
Function |
Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. |
Tissue Specificity |
Leukocytes. |
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Belongs to the integrin alpha chain family. |
Aliases |
Antigen CD11A (p180), lymphocyte function associated antigen 1, alpha polypeptide antibody|Antigen CD11A antibody|CD 11a antibody|CD11 antigen-like family member A antibody|CD11a antibody|CD11a antigen antibody|Integrin Alpha L antibody|Integrin alpha-L antibody|Integrin gene promoter antibody|Integrin, alpha L (antigen CD11A (p180), lymphocyte function associated antigen 1; alpha polypeptide) antibody|ITAL_HUMAN antibody|ITGAL antibody|ITGAL protein antibody|Leukocyte adhesion glycoprotein LFA 1 alpha chain antibody|Leukocyte adhesion glycoprotein LFA-1 alpha chain antibody|Leukocyte Adhesion Glycoprotein LFA1 Alpha Chain antibody|Leukocyte function associated molecule 1 alpha chain antibody|Leukocyte function-associated molecule 1 alpha chain antibody|LFA 1 alpha antibody|LFA 1 antibody|LFA 1A antibody|LFA-1A antibody|LFA1A antibody|Ly15 antibody|Ly21 antibody|Lymphocyte function associated antigen 1 antibody|Lymphocyte Function Associated Antigen Type 1 alpha antibody|p180 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD11a antibody, ASA-B0343–1.jpg
All lanes: Anti CD11A (ASA-B0343) at 0.5ug/ml
WB: Recombinant Human CD11A Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
All lanes: Anti CD11A (ASA-B0343) at 0.5ug/ml
WB: Recombinant Human CD11A Protein 0.5ng
Predicted bind size: 45KD
Observed bind size: 45KD
Anti-CD11a antibody, ASA-B0343–2.jpg
All lanes: Anti CD11A (ASA-B0343) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 129KD
Observed bind size: 129KD
All lanes: Anti CD11A (ASA-B0343) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: CEM Whole Cell Lysate at 40ug
Predicted bind size: 129KD
Observed bind size: 129KD
