Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| hyaluronan-mediated motility receptor(RHAMM) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CD168(706-724aa KEGNTNCYRAPMECQESWK). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
HMMR |
Protein |
Hyaluronan mediated motility receptor |
Uniprot ID |
O75330 |
Function |
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular- regulated kinase (ERK) activity. |
Tissue Specificity |
|
Sub-cellular localization |
Cell surface. Cytoplasm . |
Sequence Similarities |
|
Aliases |
CD168 antibody|CD168 antigen antibody|HMMR antibody|HMMR_HUMAN antibody|Hyaluronan mediated motility receptor antibody|Hyaluronan-mediated motility receptor(RHAMM) antibody|IHABP antibody|Intracellular hyaluronic acid-binding protein antibody|MGC119494 antibody|MGC119495 antibody|OTTHUMP00000196920 antibody|Receptor for hyaluronan-mediated motility antibody|RHAMM antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD168 antibody, ASA-B0357, Western blotting
Lane 1: MM231 Cell Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Lane 1: MM231 Cell Lysate
Lane 2: MM453 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: A549 Cell Lysate
Anti-CD168 antibody, ASA-B0357, IHC(P)
IHC(P): Human Placenta Tissue
IHC(P): Human Placenta Tissue
