Description
Data Sheet
| Antibody Properties | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Available Conjugates | Staining antibody. For staining, use 10ul/1,000,000 cells. |
| Biological Activity | Fully biologically active when compared to standard determined by the apoptosis assay of Raji cell in vitro. |
| Description | Recombinant Anti Human CD20 monoclonal Antibody produced in CHO is a glycosylated polypeptide chain having a molecular mass of 80 kDa.CD-20 is purified by proprietary chromatographic techniques. |
| Expression host | CHO. |
| Immunological function | This antibody is also available unconjugated to and conjugated to Biotin. For staining with biotin or FITC-conjugated antibody use 5-10ul/1,000,000 cells. |
| Protein Background | CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. CD20 plays a role in the development and differentiation of B-cells into plasma cells. CD20 is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein. |
| Purity | Greater than 95.0% as determined by:Analysis by RP-HPLC.Analysis by SDS-PAGE gel. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Solubility | It is recommended to reconstitute the lyophilized rAHuCD-20 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized CD-20 Recombinant Antibody although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD20 Antibody should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | B1, S7, Bp35, MS4A2, LEU-16, MGC3969. |
| Type | Anti Human Recombinant. |
