Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| CD80 molecule | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human CD80(57-71aa EELAQTRIYWQKEKK). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CD80 |
Protein |
T-lymphocyte activation antigen CD80 |
Uniprot ID |
P33681 |
Function |
Involved in the costimulatory signal essential for T- lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation. |
Tissue Specificity |
Expressed on activated B-cells, macrophages and dendritic cells. |
Sub-cellular localization |
Membrane; Single-pass type I membrane protein. |
Sequence Similarities |
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Aliases |
Activation B7 1 antigen antibody|Activation B7-1 antigen antibody|Activation B71 antigen antibody|B lymphocyte activation antigen B7 antibody|B7 antibody|B7-1 antibody|B7-1 antigen antibody|BB1 antibody|CD 80 antibody|CD28 antigen ligand 1 antibody|CD28 antigen ligand 1 B7 1 antigen antibody|CD28 antigen ligand 1 B71 antigen antibody|CD28LG antibody|CD28LG1 antibody|CD80 antibody|CD80 antigen antibody|CD80 antigen precursor antibody|CD80 molecule antibody|CD80_HUMAN antibody|Costimulatory factor CD80 antibody|CTLA 4 counter receptor B7.1 antibody|CTLA-4 counter-receptor B7.1 antibody|LAB 7 antibody|LAB7 antibody|T lymphocyte activation antigen CD80 antibody|T-lymphocyte activation antigen CD80 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD80 antibody, ASA-B0406, Western blotting
Lane 1: Recombinant Human CD80 Protein 10ng
Lane 2: Recombinant Human CD80 Protein 5ng
Lane 3: Recombinant Human CD80 Protein 2.5ng
Lane 1: Recombinant Human CD80 Protein 10ng
Lane 2: Recombinant Human CD80 Protein 5ng
Lane 3: Recombinant Human CD80 Protein 2.5ng
