Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| CD86 molecule | Polyclonal | IgG | Rabbit | Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse CD86(66-80aa YEHYLGTEKLDSVNA), different from the related rat sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CD86 |
Protein |
T-lymphocyte activation antigen CD86 |
Uniprot ID |
P42082 |
Function |
Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. |
Tissue Specificity |
Expressed on activated B-cells. |
Sub-cellular localization |
Cell membrane ; Single-pass type I membrane protein . |
Sequence Similarities |
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. |
Aliases |
Activation B7-2 antigen 3 antibody|Activation B7-2 antigen antibody|B-lymphocyte activation antigen B7-2 2 antibody|B-lymphocyte activation antigen B7-2 antibody|B7 2 antibody|B7 antibody|B7-2 antibody|B7.2 antibody|B70 antibody|B72 antigen antibody|BU63 antibody|CD28 antigen ligand 2 2 antibody|CD28 antigen ligand 2 antibody|Cd28l2 antibody|CD28LG2 antibody|CD86 antibody|CD86 antigen(CD28 antigen ligand 2, B7-2 antigen) 1, 2 antibody|CD86 antigen(CD28 antigen ligand 2, B7-2 antigen) antibody|CD86 antigen antibody|CD86 molecule antibody|CD86_HUMAN antibody|CLS1 antibody|CTLA-4 counter-receptor B7.2 2, 3 antibody|CTLA-4 counter-receptor B7.2 antibody|Early T-cell costimulatory molecule 1 antibody|ETC-1 antibody|FUN 1 antibody|FUN-1 antibody|LAB72 antibody|Ly-58 antibody|Ly58 antibody|MB7 antibody|MB7-2 antibody|Membrane glycoprotein antibody|MGC34413 antibody|T-lymphocyte activation antigen CD86 antibody|TS/A-2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CD86 antibody, ASA-B0409, Western blotting
Lane 1: Recombinant Mouse CD86 Protein 10ng
Lane 2: Recombinant Mouse CD86 Protein 5ng
Lane 3: Recombinant Mouse CD86 Protein 2.5ng
Lane 1: Recombinant Mouse CD86 Protein 10ng
Lane 2: Recombinant Mouse CD86 Protein 5ng
Lane 3: Recombinant Mouse CD86 Protein 2.5ng
