Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cell division cycle 20 | Polyclonal | IgG | Rabbit | Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cdc20(20-36aa IPNAPVARWQRKAKEAT), different from the related rat sequence by one amino acid, and from the related human sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CDC20 |
Protein |
Cell division cycle protein 20 homolog |
Uniprot ID |
Q9JJ66 |
Function |
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle pole . |
Sequence Similarities |
Belongs to the WD repeat CDC20/Fizzy family. |
Aliases |
bA276H19.3 antibody|Cdc 20 antibody|CDC20 antibody|CDC20 cell division cycle 20 homolog antibody|CDC20_HUMAN antibody|CDC20A antibody|Cell division cycle 20 antibody|Cell division cycle 20 homolog(S. cerevisiae) antibody|Cell division cycle 20 homolog antibody|Cell division cycle protein 20 homolog antibody|fizzy antibody|MGC102824 antibody|p55CDC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cdc20 antibody, ASA-B0414, Western blotting
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
Lane 1: Rat Thymus Tissue Lysate
Lane 2: Rat Spleen Tissue Lysate
