Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cofilin 1 (non-muscle) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | ELISA, IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Cofilin recombinant protein (Position: A2-L166). Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CFL1 |
Protein |
Cofilin-1 |
Uniprot ID |
P23528 |
Function |
Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. |
Tissue Specificity |
Widely distributed in various tissues. |
Sub-cellular localization |
Nucleus matrix. |
Sequence Similarities |
Belongs to the actin-binding proteins ADF family. |
Aliases |
18 kDa phosphoprotein antibody|CFL 1 antibody|CFL antibody|CFL1 antibody|COF1_HUMAN antibody|Cofilin 1 antibody|Cofilin 1 non muscle antibody|Cofilin antibody|Cofilin non muscle isoform antibody|Cofilin-1 antibody|non-muscle isoform antibody|p18 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
| ELISA | 0.1-0.5μg/ml | Human | Sandwich ELISA format |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cofilin antibody, ASA-B0466–1.jpg
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-Cofilin antibody, ASA-B0466–2.jpg
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Cofilin antibody, ASA-B0466–3.jpg
IHC(P): Mouse Lung Tissue
IHC(P): Mouse Lung Tissue
