Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| gap junction protein, alpha 4, 37kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Connexin 37(3-17aa DWGFLEKLLDQVQEH), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
GJA4 |
Protein |
Gap junction alpha-4 protein |
Uniprot ID |
P35212 |
Function |
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
Tissue Specificity |
Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium. |
Sub-cellular localization |
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities |
|
Aliases |
Connexin 37 antibody|Connexin-37 antibody|Cx37 antibody|CXA4_HUMAN antibody|Gap junction alpha 4 protein antibody|Gap junction alpha-4 protein antibody|gap junction protein, alpha 4 37kDa(connexin 37) antibody|gap junction protein, alpha 4, 37kD(connexin 37) antibody|Gap junction protein, alpha 4, 37kDa antibody|Gja4 antibody|OTTHUMP00000004181 antibody|OTTHUMP00000004182 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Connexin 37/GJA4 antibody, ASA-B0482, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: NIH3T3 Cell Lysate
Lane 5: SKOV Cell Lysate
Lane 6: A431 Cell Lysate
Lane 1: Rat Heart Tissue Lysate
Lane 2: HELA Cell Lysate
Lane 3: MCF-7 Cell Lysate
Lane 4: NIH3T3 Cell Lysate
Lane 5: SKOV Cell Lysate
Lane 6: A431 Cell Lysate
Anti-Connexin 37/GJA4 antibody, ASA-B0482, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
Anti-Connexin 37/GJA4 antibody, ASA-B0482, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Rat Cardiac Muscle Tissue
