Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| gap junction protein, gamma 1, 45kDa |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 45(308-322aa KIAYKQNKANTAQEQ), different from the related mouse and rat sequences by one amino acid. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
GJC1 |
Protein
|
Gap junction gamma-1 protein |
Uniprot ID
|
P36383 |
Function
|
One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. |
Tissue Specificity
|
|
Sub-cellular localization
|
Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. |
Sequence Similarities
|
|
Aliases
|
Connexin 45 antibody|Connexin-45 antibody|Connexin45 antibody|CX 31.9 antibody|CX 45 antibody|CX31.9 antibody|Cx45 antibody|CXG1_HUMAN antibody|DKFZp686P0738 antibody|Gap junction alpha 7 protein antibody|Gap junction alpha-7 protein antibody|Gap junction gamma 1 protein antibody|Gap junction gamma-1 protein antibody|Gap junction protein alpha 7 45kDa antibody|Gap junction protein alpha 7 antibody|Gap junction protein, gamma 1, 45kDa antibody|GJA 7 antibody|GJA7 antibody|GJC1 antibody|GJD3 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human Mouse, Rat |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Connexin 45/GJA7 antibody, ASA-B0486, Western blotting
WB: MCF-7 Cell Lysate
