Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| v-crk sarcoma virus CT10 oncogene homolog(avian)-like | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human CrkL(286-303aa FPFTHVKIFDPQNPDENE), different from the related mouse and rat sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CRKL |
Protein |
Crk-like protein |
Uniprot ID |
P46109 |
Function |
May mediate the transduction of intracellular signals. |
Tissue Specificity |
|
Sub-cellular localization |
|
Sequence Similarities |
Belongs to the CRK family. |
Aliases |
Crk L antibody|Crk like protein antibody|Crk-like protein antibody|Crkl antibody|CRKL_HUMAN antibody|Crkol antibody|HGNC:2363 antibody|Oncogene CrkL antibody|V crk avian sarcoma virus CT10 oncogene homolog like antibody|v crk sarcoma virus CT10 oncogene homolog(avian) like antibody|V crk sarcoma virus CT10 oncogene homolog avian like antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CrkL antibody, ASA-B0500, Western blotting
All lanes: Anti CrkL (ASA-B0500) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Lane 8: MM543 Whole Cell Lysate at 40ug
Lane 9: JURKAT Whole Cell Lysate at 40ug
Pr
All lanes: Anti CrkL (ASA-B0500) at 0.5ug/ml
Lane 1: Rat Spleen Tissue Lysate at 50ug
Lane 2: Rat Ovary Tissue Lysate at 50ug
Lane 3: Rat Testis Tissue Lysate at 50ug
Lane 4: MM231 Whole Cell Lysate at 40ug
Lane 5: A431 Whole Cell Lysate at 40ug
Lane 6: MCF-7 Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Lane 8: MM543 Whole Cell Lysate at 40ug
Lane 9: JURKAT Whole Cell Lysate at 40ug
Pr
