Cullin 2 Antibody

Cullin 2 Antibody

$330.00

In stock

SKU: ASA-B0513 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
cullin 2 Polyclonal IgG Rabbit Human, Mouse, Rat WB Immunogen affinity purified.

Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Cullin 2(264-280aa HECQQRMVADHLQFLHA), different from the related mouse and rat sequences by one amino acid.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.

Concentration

 Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 CUL2

Protein

 Cullin-2

Uniprot ID

 Q13617

Function

 Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin- conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF).

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

 Cul2 antibody|CUL 2 antibody|CUL-2 antibody|CUL2 antibody|CUL2_HUMAN antibody|cullin 2 antibody|cullin homolog 2 antibody|Cullin-2 antibody|MGC131970 antibody

Application Details

Application Concentration* Species Validated Using**
Western blot 0.1-0.5μg/ml Human, Rat Mouse AssaySolutio’s ECL kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Cullin 2 antibody, ASA-B0513, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant human Cul2, 46.5KD(162aa tag+L150-Y394)
Lane 1: Recombinant Human Cul2 Protein 10ng
Lane 2: Recombinant Human Cul2 Protein 5ng
Lane 3: Recombinant Human Cul2 Protein 2.5ng
Anti-Cullin 2 antibody, ASA-B0513, Western blotting
WB: Rat Brain Tissue Lysate
Anti-Cullin 2 antibody, ASA-B0513, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: SMMC Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: COLO320 Cell Lysate

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