Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine(C-X3-C motif) ligand 1 | Polyclonal | IgG | Rabbit | Mouse, Rat | IHC-P, IHC-F, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of mouse CX3CL1(74-92aa CADPKEKWVQDAMKHLDHQ), identical to the related rat sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CX3CL1 |
Protein |
Fractalkine |
Uniprot ID |
O35188 |
Function |
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity). Binds to CX3CR1. |
Tissue Specificity |
Highest levels in brain. Lower levels in kidney, heart and lung. Also found in skeletal muscle and testis. |
Sub-cellular localization |
Cell membrane; Single-pass type I membrane protein. |
Sequence Similarities |
|
Aliases |
A 152E5.2 antibody|AB030188 antibody|ABCD 3 antibody|ABCD3 antibody|AI848747 antibody|C-X3-C motif chemokine 1 antibody|C3Xkine antibody|Chemokine(C-X3-C motif) ligand 1 antibody|Chemokine C X3 C motif ligand 1 antibody|Chemokine CX3C Motif Ligand 1 antibody|CX3C membrane anchored chemokine antibody|CX3C membrane-anchored chemokine antibody|CX3CL1 antibody|CXC 3 antibody|CXC3 antibody|CXC3C antibody|D8Bwg0439e antibody|FKN antibody|Fractalkine antibody|Neurotactin antibody|NTN antibody|NTT antibody|Processed fractalkine antibody|SCYD 1 antibody|SCYD1 antibody|Small inducible cytokine D1 antibody|Small inducible cytokine subfamily D(Cys X3 Cys) member 1 antibody|small inducible cytokine subfamily D(Cys-X3-Cys), member 1(fractalkine, neurotactin) antibody|Small inducible cytokine subfamily D member 1 antibody|Small-inducible cytokine D1 antibody|X3CL1_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Rat | AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) | 0.5-1μg/ml | Mouse, Rat Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-CX3CL1 antibody, ASA-B0518, Western blotting
Lane 1: Recombinant Mouse Fractalkin Protein 10ng
Lane 2: Recombinant Mouse Fractalkin Protein 5ng
Lane 3: Recombinant Mouse Fractalkin Protein 2.5ng
Lane 1: Recombinant Mouse Fractalkin Protein 10ng
Lane 2: Recombinant Mouse Fractalkin Protein 5ng
Lane 3: Recombinant Mouse Fractalkin Protein 2.5ng
Anti-CX3CL1 antibody, ASA-B0518, IHC(P)
IHC(P): Rat Intestine Tissue
IHC(P): Rat Intestine Tissue
