Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cyclin B1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCNB |
Protein |
G2/mitotic-specific cyclin-B1 |
Uniprot ID |
P14635 |
Function |
Essential for the control of the cell cycle at the G2/M (mitosis) transition. |
Tissue Specificity |
|
Sub-cellular localization |
Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. |
Sequence Similarities |
Belongs to the cyclin family. Cyclin AB subfamily. |
Aliases |
CCNB 1 antibody|CCNB antibody|ccnb1 antibody|CCNB1_HUMAN antibody|G2 mitotic specific cyclin B1 antibody|G2/mitotic-specific cyclin-B1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cyclin B1 antibody, ASA-B0529–1.jpg
All lanes: Anti Cyclin B1 (ASA-B0529) at 0.5ug/ml
WB: Recombinant Human Cyclin B1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti Cyclin B1 (ASA-B0529) at 0.5ug/ml
WB: Recombinant Human Cyclin B1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti-Cyclin B1 antibody, ASA-B0529–2.jpg
All lanes: Anti Cyclin B1 (ASA-B0529) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 56KD
All lanes: Anti Cyclin B1 (ASA-B0529) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: 293T Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 48KD
Observed bind size: 56KD
