Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cyclin D3 | Polyclonal | IgG | Rabbit | Human, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCND3 |
Protein |
G1/S-specific cyclin-D3 |
Uniprot ID |
P30281 |
Function |
Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the cyclin family. Cyclin D subfamily. |
Aliases |
CCND 3 antibody|CCND3 antibody|CCND3_HUMAN antibody|CyclinD3 antibody|D3 type cyclin antibody|G1 S specific cyclin D3 antibody|G1/S specific cyclin D3 antibody|G1/S-specific cyclin-D3 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cyclin D3 antibody, ASA-B0537–1.jpg
All lanes: Anti Cyclin D3 (ASA-B0537) at 0.5ug/ml
WB: Recombinant Human Cyclin D3 Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD
All lanes: Anti Cyclin D3 (ASA-B0537) at 0.5ug/ml
WB: Recombinant Human Cyclin D3 Protein 0.5ng
Predicted bind size: 25KD
Observed bind size: 25KD
Anti-Cyclin D3 antibody, ASA-B0537–2.jpg
All lanes: Anti Cyclin D3 (ASA-B0537) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Lane 4: Rat Ovary Tissue Lysate at 50ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bi
All lanes: Anti Cyclin D3 (ASA-B0537) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: Rat Lung Tissue Lysate at 50ug
Lane 4: Rat Ovary Tissue Lysate at 50ug
Lane 5: JURKAT Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Lane 7: MCF-7 Whole Cell Lysate at 40ug
Lane 8: HELA Whole Cell Lysate at 40ug
Predicted bind size: 33KD
Observed bi
