Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| cytochrome P450, family 2, subfamily E, polypeptide 1 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human CYP2E1 recombinant protein (Position: M1-Y310). Human CYP2E1 shares 73% and 74% amino acid (aa) sequences identity with mouse and rat CYP2E1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CYP2E1 |
Protein |
Cytochrome P450 2E1 |
Uniprot ID |
P05181 |
Function |
Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites. Inactivates a number of drugs and xenobiotics and also bioactivates many xenobiotic substrates to their hepatotoxic or carcinogenic forms. |
Tissue Specificity |
|
Sub-cellular localization |
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. |
Sequence Similarities |
Belongs to the cytochrome P450 family. |
Aliases |
4-nitrophenol 2-hydroxylase antibody|CP2E1_HUMAN antibody|CPE 1 antibody|CPE1 antibody|CYP2E antibody|CYP2E1 antibody|CYPIIE1 antibody|Cytochrome P450 2E1 antibody|cytochrome P450 family 2 subfamily E polypeptide 1 antibody|Cytochrome P450 isozyme 3A antibody|Cytochrome P450 LM3A antibody|Cytochrome P450 subfamily IIE (ethanol inducible) polypeptide 1 antibody|Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1 antibody|Cytochrome P450, subfamily IIE antibody|Cytochrome P450-ALC antibody|Cytochrome P450-J antibody|Cytochrome P450RLM6 antibody|EC 1.14.13.- antibody|EC 1.14.13.n7 antibody|Ethanol inducible p450 antibody|Ethanol-inducible P450 antibody|flavoprotein-linked monooxygenase antibody|MGC133529 antibody|microsomal monooxygenase antibody|OTTHUMP00000020813 antibody|OTTHUMP00000046571 antibody|P450 ALC antibody|P450 J antibody|P450-J antibody|P450C2E antibody|P450J antibody|P450RLM6 antibody|xenobiotic monooxygenase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Mouse, Rat Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- CYP2E1 antibody, ASA-B0549, Western blotting
All lanes: Anti CYP2E1 (ASA-B0549) at 0.5ug/ml
WB: Recombinant Human CYP2E1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
All lanes: Anti CYP2E1 (ASA-B0549) at 0.5ug/ml
WB: Recombinant Human CYP2E1 Protein 0.5ng
Predicted bind size: 39KD
Observed bind size: 39KD
Anti- CYP2E1 antibody, ASA-B0549, Western blotting
All lanes: Anti CYP2E1 (ASA-B0549) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 65KD
All lanes: Anti CYP2E1 (ASA-B0549) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 65KD
Anti- CYP2E1 antibody, ASA-B0549, IHC(P)
IHC(P): Mouse Liver Tissue
IHC(P): Mouse Liver Tissue
