Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| keratin 7 |
Monoclonal |
IgG |
Mouse |
Human |
IHC-P, IHC-F, WB |
Ascites |
Immunogen
|
| Cytoskeletal preparation of the RT4 human bladder carcinoma cell line. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request. |
Concentration
|
1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml. |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
KRT7 |
Protein
|
Keratin, type II cytoskeletal 7 |
Uniprot ID
|
Human(P08729), Mouse(Q9DCV7), Rat(Q6IG12) |
Function
|
Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). |
Tissue Specificity
|
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus. |
Sub-cellular localization
|
Cytoplasm. |
Sequence Similarities
|
Belongs to the intermediate filament family. |
Aliases
|
SCL|cytokeratin 7|K2C7|K7|keratin|Keratin-7|55K type II cytoskeletal|keratin, type II cytoskeletal 7|sarcolectin|KERATIN, SIMPLE EPITHELIAL|KERATIN, TYPE II, CYTOSKELETAL, 7|SARCOLECTIN|Type-II keratin Kb7 |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
1-2μg/ml |
Human |
AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) |
2-4μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
| Immunohistochemistry(Frozen Section) |
2-4μg/ml |
Human |
AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Mouse Chemiluminescent WB Detection Kit (AKIT003B) for Western blot, and Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information
