Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| DEAD(Asp-Glu-Ala-Asp) box helicase 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DDX5(566-580aa TYQNGYDSTQQYGSN), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DDX5 |
Protein |
Probable ATP-dependent RNA helicase DDX5 |
Uniprot ID |
P17844 |
Function |
Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for estrogen receptor ESR1 and androgen receptor AR. Increases ESR1 AF-1 domain-mediated transactivation and ESR1 AF-1 and AF-2 domains transcriptional synergistic activity. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specicic manner; the function probbaly involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3′ transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus, nucleolus. |
Sequence Similarities |
Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily. |
Aliases |
ATP dependent RNA helicase DDX5 antibody|DDX 5 antibody|Ddx5 antibody|DDX5_HUMAN antibody|DEAD(Asp Glu Ala Asp) box helicase 5 antibody|DEAD(Asp Glu Ala Asp) box polypeptide 5 antibody|DEAD box 5 antibody|DEAD box protein 5 antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5(RNA helicase, 68kD) antibody|DEAD/H(Asp Glu Ala Asp/His) box polypeptide 5 antibody|DEAD/H(Asp-Glu-Ala-Asp/His) box polypeptide 5(RNA helicase 68kDa) antibody|DEAD/H box-5(RNA helicase 68kD) antibody|DKFZp686J01190 antibody|G17P1 antibody|HELR antibody|HLR 1 antibody|HLR1 antibody|HUMP68 antibody|P68 antibody|p68 RNA helicase antibody|Probable ATP dependent RNA helicase DDX5 antibody|Probable ATP-dependent RNA helicase DDX5 antibody|RNA helicase 68kD antibody|RNA helicase p68 antibody|RNA-dependent ATPase antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DDX5 antibody, ASA-B0581, Western blotting
All lanes: Anti DDX5 (ASA-B0581) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
All lanes: Anti DDX5 (ASA-B0581) at 0.5ug/ml
Lane 1: Rat Testis Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Lane 4: MCF-7 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Lane 6: SKOV Whole Cell Lysate at 40ug
Lane 7: MM231 Whole Cell Lysate at 40ug
Predicted bind size: 69KD
Observed bind size: 69KD
Anti-DDX5 antibody, ASA-B0581, IHC(P)
IHC(P): Rat Brain Tissue
IHC(P): Rat Brain Tissue
