Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| desmoglein 3 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 3(981-999aa QLRGSHTMLCTEDPCSRLI ). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DSG3 |
Protein |
Desmoglein-3 |
Uniprot ID |
P32926 |
Function |
Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. |
Tissue Specificity |
Epidermis, tongue, tonsil, esophagus and carcinomas. |
Sub-cellular localization |
Cell membrane ; Single-pass type I membrane protein . Cell junction, desmosome . |
Sequence Similarities |
|
Aliases |
130 kD pemphigus vulgaris antigen antibody|130 kDa pemphigus vulgaris antigen antibody|Bal antibody|Balding antibody|Cadherin family member 6 antibody|CDHF 6 antibody|CDHF6 antibody|Desmoglein 3 antibody|Desmoglein 3 precursor antibody|Desmoglein3 antibody|DKFZp686P23184 antibody|DSG 3 antibody|DSG3 antibody|Pemphigus vulgaris antigen antibody|PVA antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Desmoglein 3 antibody, ASA-B0588, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: CEM Cell Lysate
Lane 1: A431 Cell Lysate
Lane 2: CEM Cell Lysate
