Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| delta-like 3(Drosophila) | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DLL3(599-618aa RAGQRQHLLFPYPSSILSVK). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DLL3 |
Protein |
Delta-like protein 3 |
Uniprot ID |
Q9NYJ7 |
Function |
Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Membrane ; Single-pass type I membrane protein . |
Sequence Similarities |
Contains 1 DSL domain. |
Aliases |
Delta Drosophila like 3 antibody|Delta like 3 Drosophila antibody|Delta like 3 homolog Drosophila antibody|Delta like 3 protein antibody|Delta like protein 3 precursor antibody|Delta-like protein 3 antibody|Delta3 antibody|Dll3 antibody|DLL3_HUMAN antibody|Drosophila Delta homolog 3 antibody|SCDO1 antibody|SCOD1 antibody|Spondylocostal dysostosis autosomal recessive antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DLL3 antibody, ASA-B0599, Western blotting
All lanes: Anti DLL3 (ASA-B0599) at 0.5ug/ml
Lane 1: Recombinant Human DLL3 Protein 10ng
Lane 2: Recombinant Human DLL3 Protein 5ng
Lane 3: Recombinant Human DLL3 Protein 2.5ng
Lane 4: Recombinant Human DLL3 Protein 1.25ng
Predicted bind size: 41KD
Observed bind size: 41KD
All lanes: Anti DLL3 (ASA-B0599) at 0.5ug/ml
Lane 1: Recombinant Human DLL3 Protein 10ng
Lane 2: Recombinant Human DLL3 Protein 5ng
Lane 3: Recombinant Human DLL3 Protein 2.5ng
Lane 4: Recombinant Human DLL3 Protein 1.25ng
Predicted bind size: 41KD
Observed bind size: 41KD
