Description
Overview
Long Name
|
Antibody Type
|
Antibody Isotype
|
Host
|
Species Reactivity
|
Validated Applications
|
Purification
|
| dihydropyrimidine dehydrogenase |
Polyclonal |
IgG |
Rabbit |
Human, Mouse, Rat |
WB |
Immunogen affinity purified. |
Immunogen
|
| A synthetic peptide corresponding to a sequence at the N-terminus of human DPYD(33-52aa AKKLDKKHWKRNPDKNCFNC), different from the related rat and mouse sequences by one amino acid. |
Properties
Form
|
Lyophilized |
Size
|
100 µg/vial |
Contents
|
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration
|
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage
|
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene
|
DPYD |
Protein
|
Dihydropyrimidine dehydrogenase [NADP(+)](DHPDHase/DPD) |
Uniprot ID
|
Q12882 |
Function
|
Involved in pyrimidine base degradation. Catalyzes the reduction of uracil and thymine. Also involved the degradation of the chemotherapeutic drug 5-fluorouracil. |
Tissue Specificity
|
Found in most tissues with greatest activity found in liver and peripheral blood mononuclear cells. |
Sub-cellular localization
|
Cytoplasm. |
Sequence Similarities
|
Belongs to the dihydropyrimidine dehydrogenase family. |
Aliases
|
DHP antibody|DHPDHase antibody|Dihydropyrimidine dehydrogenase[NADP(+)] antibody|Dihydropyrimidine dehydrogenase[NADP+] antibody|Dihydropyrimidine dehydrogenase antibody|Dihydrothymine dehydrogenase antibody|Dihydrouracil dehydrogenase antibody|DPD antibody|DPYD antibody|DPYD_HUMAN antibody|MGC132008 antibody|MGC70799 antibody|OTTHUMP00000058954 antibody |
Application Details
| Application |
Concentration* |
Species |
Validated Using** |
| Western blot |
0.1-0.5μg/ml |
Human Mouse, Rat |
AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DPYD antibody, ASA-B0607, Western blotting
WB: MM231 Cell Lysate
