Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| dual specificity phosphatase 3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human DUSP3(155-171aa RQNREIGPNDGFLAQLC), identical to the related rat and mouse sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
DUSP3 |
Protein |
Dual specificity protein phosphatase 3 |
Uniprot ID |
P51452 |
Function |
Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2. |
Tissue Specificity |
|
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. |
Aliases |
Dual specificity phosphatase 3 antibody|Dual specificity protein phosphatase 3 antibody|Dual specificity protein phosphatase VHR antibody|DUS3_HUMAN antibody|DUSP3 antibody|Serine/threonine specific protein phosphatase antibody|Vaccinia H1-related phosphatase antibody|Vaccinia virus phosphatase VH1 related antibody|VHR antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-DUSP3 antibody, ASA-B0612, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: SKOV Cell Lysate
Lane 3: MM453 Cell Lysate
Lane 1: Rat Testis Tissue Lysate
Lane 2: SKOV Cell Lysate
Lane 3: MM453 Cell Lysate
