Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| FXYD domain containing ion transport regulator 5 | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Dysadherin(165-188aa KCRQLSRLCRNRCR). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FXYD5 |
Protein |
FXYD domain-containing ion transport regulator 5 |
Uniprot ID |
Q96DB9 |
Function |
Involved in down-regulation of E-cadherin which results in reduced cell adhesion. Promotes metastasis. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane ; Single-pass type I membrane protein . |
Sequence Similarities |
Belongs to the FXYD family. |
Aliases |
Dysad antibody|Dysadherin antibody|FXYD 5 antibody|FXYD domain containing ion transport regulator 5 antibody|FXYD domain-containing ion transport regulator 5 antibody|Fxyd5 antibody|FXYD5_HUMAN antibody|FXYD5RIC antibody|HSPC 113 antibody|HSPC113 antibody|IWU 1 antibody|IWU1 antibody|KCT 1 antibody|KCT1 antibody|Keratinocytes associated transmembrane protein 1 antibody|OIT 2 antibody|OIT2 antibody|PRO 6241 antibody|PRO6241 antibody|RIC antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FXYD5 antibody, ASA-B0614, Western blotting
All lanes: Anti FXYD5 (ASA-B0614) at 0.5ug/ml
WB: Rat PancreasTissue Lysate at 50ug
Predicted bind size: 19KD
Observed bind size: 19KD
All lanes: Anti FXYD5 (ASA-B0614) at 0.5ug/ml
WB: Rat PancreasTissue Lysate at 50ug
Predicted bind size: 19KD
Observed bind size: 19KD
Anti- FXYD5 antibody, ASA-B0614, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
