Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| E2F transcription factor 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human E2F2(422-427aa ISDLFDSYDLGDLLIN), identical to the related mouse sequence. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
E2F2 |
Protein |
Transcription factor E2F2(E2F-2) |
Uniprot ID |
Q14209 |
Function |
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5′-TTTC[CG]CGC- 3′ found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. |
Tissue Specificity |
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Belongs to the E2F/DP family. |
Aliases |
dE2F2 antibody|E2F transcription factor 2 antibody|E2F-2 antibody|E2F2 antibody|E2F2_HUMAN antibody|Transcription factor E2F2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-E2F2 antibody, ASA-B0620, Western blotting
All lanes: Anti E2F2 (ASA-B0620) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: SMMC Whole Cell Lysate at 40ug
Predi
All lanes: Anti E2F2 (ASA-B0620) at 0.5ug/ml
Lane 1: Rat Lung Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Rat Kidney Tissue Lysate at 50ug
Lane 5: HELA Whole Cell Lysate at 40ug
Lane 6: COLO320 Whole Cell Lysate at 40ug
Lane 7: A549 Whole Cell Lysate at 40ug
Lane 8: MCF-7 Whole Cell Lysate at 40ug
Lane 9: SMMC Whole Cell Lysate at 40ug
Predi
