Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| solute carrier family 1 (glial high affinity glutamate transporter), member 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human EAAT2 recombinant protein (Position: T461-K574). Human EAAT2 shares 96% amino acid (aa) sequence identity with both mouse and rat EAAT2. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
SLC1A2 |
Protein |
Excitatory amino acid transporter 2 |
Uniprot ID |
P43004 |
Function |
Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. |
Tissue Specificity |
|
Sub-cellular localization |
Membrane; Multi-pass membrane protein. |
Sequence Similarities |
|
Aliases |
EAA2_HUMAN antibody|EAAT2 antibody|Excitatory amino acid transporter 2 antibody|Excitotoxic amino acid transporter 2 antibody|Glial high affinity glutamate transporter antibody|GLT 1 antibody|GLT1 antibody|Glutamate aspartate transporter II antibody|Glutamate transporter 1 antibody|Glutamate/aspartate transporter II antibody|Slc1a2 antibody|Sodium dependent glutamate aspartate transporter 2 antibody|Sodium-dependent glutamate/aspartate transporter 2 antibody|solute carrier family 1 (glial high affinity glutamate transporter), member 2 antibody|Solute carrier family 1 glial high affinity glutamate transporter member 2 antibody|Solute carrier family 1 member 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EAAT2 antibody, ASA-B0626, Western blotting
All lanes: Anti EAAT2 (ASA-B0626) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 62KD
Observed bind size: 62KD
All lanes: Anti EAAT2 (ASA-B0626) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: SMMC Whole Cell Lysate at 40ug
Lane 5: PANC Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 62KD
Observed bind size: 62KD
