Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence at the C-terminus of human EIF2S2(199-214aa FQAVTGKRAQLRAKAN), identical to the related mouse and rat sequences. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EIF2S2 |
Protein |
Eukaryotic translation initiation factor 2 subunit 2 |
Uniprot ID |
P20042 |
Function |
eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
Tissue Specificity |
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Sub-cellular localization |
|
Sequence Similarities |
|
Aliases |
DKFZp686L18198 antibody|eIF 2 beta antibody|eIF-2-beta antibody|EIF2 antibody|EIF2B antibody|EIF2beta antibody|EIF2S2 antibody|Eukaryotic initiation factor 2 beta antibody|eukaryotic initiation factor 2-beta antibody|Eukaryotic translation initiation factor 2 beta antibody|Eukaryotic translation initiation factor 2 subunit 2 antibody|Eukaryotic translation initiation factor 2 subunit 2 beta 38kDa antibody|Eukaryotic translation initiation factor 2 subunit 2 beta antibody|Eukaryotic translation initiation factor 2 subunit beta antibody|eukaryotic translation initiation factor 2, subunit 2 beta, 38kDa antibody|IF2B_HUMAN antibody|MGC8508 antibody|PPP1R67 antibody|protein phosphatase 1, regulatory subunit 67 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-EIF2S2 antibody, ASA-B0641, Western blotting
Lane 1: M451 Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4:293T Cell Lysate
Lane 1: M451 Cell Lysate
Lane 2: JURKAT Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4:293T Cell Lysate
