Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| eukaryotic translation initiation factor 6 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human EIF6 recombinant protein (Position: N66-T210). Human EIF6 shares 99.3% amino acid (aa) sequence identity with both mouse and rat EIF6. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
EIF6 |
Protein |
Eukaryotic translation initiation factor 6 |
Uniprot ID |
P56537 |
Function |
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. |
Tissue Specificity |
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). |
Sub-cellular localization |
Cytoplasm. Nucleus, nucleolus. Note: Shuttles between cytoplasm and nucleus/nucleolus. |
Sequence Similarities |
|
Aliases |
b(2)gcn antibody|B(2)GCN homolog antibody|B4 integrin interactor antibody|Binding protein of beta-4 integrin antibody|CAB antibody|eIF-6 antibody|EIF3A antibody|EIF6 antibody|Eukaryotic translation initiation factor 3A antibody|Eukaryotic translation initiation factor 6 antibody| IF6_HUMAN antibody|ITGB4BP antibody|OK/SW-cl.27 antibody|p27 beta 4 integrin binding protein antibody|p27(BBP) antibody|p27BBP antibody|RP4-614O4.1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Mouse, Rat | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- EIF6 antibody, ASA-B0643, Western blotting
All lanes: Anti EIF6 (ASA-B0643) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
All lanes: Anti EIF6 (ASA-B0643) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Mouse Liver Tissue Lysate at 50ug
Lane 4: Human Placenta Tissue Lysate at 50ug
Lane 5: COLO320 Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 27KD
Observed bind size: 27KD
Anti- EIF6 antibody, ASA-B0643,IHC(P)
IHC(P): Mouse Intestine Tissue
IHC(P): Mouse Intestine Tissue
Anti- EIF6 antibody, ASA-B0643,IHC(P)
IHC(P): Rat Testis Tissue
IHC(P): Rat Testis Tissue
