Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MEVEKSSDGP GAAQEPTWLT DVPAAMEFIA ATEVAVIGFF QDLEIPAVPI LHSMVQKFPG VSFGISTDSE VLTHYNITGN TICLFRLVDN EQLNLEDEDI ESIDATKLSR FIEINSLHMV TEYNPVTVIG LFNSVIQIHL LLIMNKASPE YEENMHRYQK AAKLFQGKIL FILVDSGMKE NGKVISFFKL KESQLPALAI YQTLDDEWDT LPTAEVSVEH VQNFCDGFLS GKLLKENRES EGKTPKVEL. |
| Description | ERP27 Human Recombinant fused with a 21 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 269 amino acids (26-273 a.a.) and having a molecular mass of 30kDa. The ERP27 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The ERP27 solution (1 mg/ml) contains 20mM Tris buffer(pH 8.0), 10% glycerol and 1mM DTT. |
| Protein Background | Endoplasmic reticulum protein 27kDa (ERP27) is a widely expressed protein which localizes to the ER and may act as a protease, protein disulfide isomerase, thiol-disulfide oxidase or phospholipase. ERP27 doesnt contain a CXXC active site motif indicating that it is a catalytically redox-inactive member of the protein disulfide isomerase family. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | ERP27 should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Synonyms | Endoplasmic reticulum resident protein 27, ER protein 27, ERp27, ERP27, PDIA8, C12orf46, FLJ32115. |
