Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| chemokine(C-C motif) ligand 11 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Eotaxin(73-92aa CADPKKKWVQDSMKYLDQKS). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
CCL11 |
Protein |
Eotaxin |
Uniprot ID |
P51671 |
Function |
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. |
Tissue Specificity |
|
Sub-cellular localization |
Secreted. |
Sequence Similarities |
Belongs to the intercrine beta (chemokine CC) family. |
Aliases |
C-C motif chemokine 11 antibody|CCL 11 antibody|CCL11 antibody|CCL11_HUMAN antibody|chemokine(C-C motif) ligand 11 antibody|Chemokine C C motif ligand 11 antibody|Chemokine CC Motif Ligand 11 antibody|Chemokine ligand 11 antibody|Eosinophil Chemotactic Protein antibody|Eotaxin antibody|eotaxin-1 antibody|MGC22554 antibody|SCYA 11 antibody|SCYA11 antibody|Small inducible cytokine A11 antibody|Small inducible cytokine subfamily A(Cys Cys) member 11 antibody|small inducible cytokine subfamily A(Cys-Cys), member 11(eotaxin) antibody|Small inducible cytokine subfamily A member 11 antibody|Small-inducible cytokine A11 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Eotaxin antibody, ASA-B0649, Western blotting
All lanes: Anti Eotaxin(ASA-B0649) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
All lanes: Anti Eotaxin(ASA-B0649) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 11KD
Observed bind size: 11KD
