Episialin, EMA Antibody (monoclonal)

Episialin, EMA Antibody (monoclonal)

$330.00

In stock

SKU: ASA-B0660 Category:

Description

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification 
mucin 1, cell surface associated Monoclonal IgG Mouse Human IHC-P, IHC-F Ascites

Immunogen
Human milk fat globule membranes.

Properties

Form

 Lyophilized

Size

 100 µg/vial

Contents

 Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative. *carrier free antibody available upon request.

Concentration

 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100 µg/ml.

Storage

 At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene

 MUC1, MUC1

Protein

 Mucin-1

Uniprot ID

 Human(15941), Mouse(02496), Rat(B2GV31)

Function

 The alpha subunit has cell adhesive properties. Can act both as an adhesion and an anti-adhesion protein. May provide a protective layer on epithelial cells against bacterial and enzyme attack.

Tissue Specificity

Expressed on the apical surface of epithelial cells, especially of airway passages, breast and uterus. Also expressed in activated and unactivated T-cells. Overexpressed in epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. Isoform 7 is expressed in tumor cells only.

Sub-cellular localization

 Apical cell membrane.

Sequence Similarities

 Contains 1 SEA domain.

Aliases

 CD227|EMA|PEM|PEMT|KL-6|PUM|MUCIN 1, URINARY|Episialin|H23AG|PEANUT-REACTIVE URINARY MUCIN|MUCIN, TUMOR-ASSOCIATED EPITHELIAL|POLYMORPHIC EPITHELIAL MUCIN|EPITHELIAL MEMBRANE ANTIGEN|mucin 1, transmembrane|Breast carcinoma-associated antigen DF3|Carcinoma-associated mucin|Krebs von den Lungen-6|Peanut-reactive urinary mucin|Polymorphic epithelial mucin|Tumor-associated epithelial membrane antigen|Tumor-associated mucin

Application Details

Application Concentration* Species Validated Using**
Immunohistochemistry(Paraffin-embedded Section) 2-4μg/ml Human AssaySolutio’s IHC/ICC Detection kit
Immunohistochemistry(Frozen Section) 2-4μg/ml Human AssaySolutio’s IHC/ICC Detection kit

AssaySolution recommends Mouse Peroxidase IHC/ICC Detection Kit (AKIT004B) for IHC(P) and IHC(F). *Blocking peptide can be purchased at $65. Contact us for more information

Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti- Episialin, EMA antibody, ASA-B0660, IHC(P)
IHC(P): Human Mammary Cancer Tissue

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