Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| fatty acid binding protein 5(psoriasis-associated) | Polyclonal | IgG | Rabbit | Human | IHC-P, WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human Fatty Acid Binding Protein 5(2-16aa ATVQQLEGRWRLVDS). | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FABP5 |
Protein |
Fatty acid-binding protein, epidermal |
Uniprot ID |
Q01469 |
Function |
High specificity for fatty acids. Highest affinity for C18 chain length. Decreasing the chain length or introducing double bonds reduces the affinity. May be involved in keratinocyte differentiation. |
Tissue Specificity |
Keratinocytes; highly expressed in psoriatic skin. |
Sub-cellular localization |
Cytoplasm. |
Sequence Similarities |
|
Aliases |
CFABP antibody|Cutaneous fatty acid binding protein antibody|DA11 antibody|Differentiation associated lipid binding protein LP2 antibody|E-FABP antibody|EFABP antibody|Epidermal-type fatty acid-binding protein antibody|FABP5 antibody|FABP5_HUMAN antibody|fatty acid binding protein 5(psoriasis-associated) antibody|Fatty acid binding protein epidermal antibody|Fatty acid-binding protein 5 antibody|Fatty acid-binding protein, epidermal antibody|Keratinocyte lipid binding protein antibody|KFABP antibody|Klbp antibody|mal1 antibody|PA-FABP antibody|PAFABP antibody|Psoriasis associated fatty acid binding protein homolog antibody|Psoriasis-associated fatty acid-binding protein homolog antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio’s IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti- Fatty Acid Binding Protein 5 antibody, ASA-B0690, Western blotting
All lanes: Anti Fatty Acid Binding Protein 5 (ASA-B0690) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 15KD
Observed bind size: 15KD
All lanes: Anti Fatty Acid Binding Protein 5 (ASA-B0690) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 15KD
Observed bind size: 15KD
Anti- Fatty Acid Binding Protein 5 antibody, ASA-B0690, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue
