Description
Data Sheet
| Amino acid sequence | TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA. |
| Biological Activity | The ED50 as calculated by the dose-dependent stimulation of the proliferation of human AML5 cells is less than 1.0 ng/ml, corresponding to a Specific Activity of 1.0106 IU/mg. |
| Description | Flt3-Ligand Human Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of approximately 17.6kDa. Flt3-Ligand is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized with no additives. |
| Protein Background | FLT3 ligand is a receptor for the fl cytokine has a tyrosine-protein kinase activity & a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand synergizes with other CSFs and interleukins to induce growth and differentiation. |
| Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | 1.Title:Identification of Y589 and Y599 in thejuxtamembrane domain of Flt3 as ligand-induced autophosphorylation sites involved in binding of Src family kinases and the protein tyrosine phosphatase SHP2.Publication:Published online before printMay 9, 2006, doi:10.1182/blood-2005-07-008896Submitted: July 14, 2005Accepted: April 26, 2006 BloodSeptember 1, 2006vol. 108no. 51542-1550.Link:http://bloodjournal.hematologylibrary.org/content/108/5/1542.full2. Title:A role of Gab2 association in Flt3 ITD mediated Stat5 phosphorylation and cell survival.Publication:Received 2 February 2009; accepted for publication 30 March 2009 Correspondence: Lars Ronnstrand,Experimental Clinical Chemistry, WallenbergLaboratory, Entr. 46, 5th fl, Malmo University Hospital, SE-20502 Malmo, Sweden.E-mail: lars.ronnstrand@med.lu.se;http://www.expklkemi.mas.lu.seLink:http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2141.2009.07725.x/pdf |
| Solubility | It is recommended to reconstitute the lyophilized Flt3-L in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Flt3-Ligand should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3. |
