Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| flavin containing monooxygenase 5 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the N-terminus of human FMO5(77-90aa DHYPNFMHNAQVLE), different from the related rat sequence by one amino acid, and different from the related mouse sequence by two amino acids. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FMO5 |
Protein |
Dimethylaniline monooxygenase[N-oxide-forming] 5 |
Uniprot ID |
P49326 |
Function |
In contrast with other forms of FMO it does not seem to be a drug-metabolizing enzyme. |
Tissue Specificity |
Expressed in fetal and adult liver. |
Sub-cellular localization |
Microsome membrane. Endoplasmic reticulum membrane. |
Sequence Similarities |
Belongs to the FMO family. |
Aliases |
Dimethylaniline monooxygenase[N oxide forming] 5 antibody|Dimethylaniline monooxygenase[N-oxide-forming] 5 antibody|Dimethylaniline oxidase 5 antibody|FMO 5 antibody|FMO5 antibody|FMO5_HUMAN antibody|Hepatic flavin containing monooxygenase 5 antibody|Hepatic flavin-containing monooxygenase 5 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FMO5 antibody, ASA-B0725, Western blotting
All lanes: Anti FMO5 (ASA-B0725) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 60KD
Observed bind size: 60KD
All lanes: Anti FMO5 (ASA-B0725) at 0.5ug/ml
Lane 1: Mouse Liver Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: Mouse Spleen Tissue Lysate at 50ug
Predicted bind size: 60KD
Observed bind size: 60KD
