Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| forkhead box M1 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human FOXM1 recombinant protein (Position: A411-Q763). Human FOXM1 shares 75% and 76% amino acid (aa) sequences identity with mouse and rat FOXM1, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FOXM1 |
Protein |
Forkhead box protein M1 |
Uniprot ID |
Q08050 |
Function |
Transcriptional factor regulating the expression of cell cycle genes essential for DNA replication and mitosis. Plays a role in the control of cell proliferation. Plays also a role in DNA breaks repair participating in the DNA damage checkpoint response. |
Tissue Specificity |
Expressed in thymus, testis, small intestine, colon followed by ovary. Appears to be expressed only in adult organs containing proliferating/cycling cells or in response to growth factors. Also expressed in epithelial cell lines derived from tumors. Not expressed in resting cells. Isoform 2 is highly expressed in testis. |
Sub-cellular localization |
Nucleus. |
Sequence Similarities |
Contains 1 fork-head DNA-binding domain. |
Aliases |
FKHL16 antibody|Forkhead box M1 antibody|Forkhead box protein M1 antibody|forkhead like 16 antibody|Forkhead-related protein FKHL16 antibody|FOX M1 antibody|Foxm1 antibody|FOXM1_HUMAN antibody|FOXM1B antibody|Hepatocyte nuclear factor 3 forkhead homolog 11 antibody|HFH-11 antibody|HFH11 antibody|HNF-3/fork-head homolog 11 antibody|HNF3 antibody|INS1 antibody|M phase phosphoprotein 2 antibody|M-phase phosphoprotein 2 antibody|MPHOSPH2 antibody|MPM 2 antibody|MPM-2 reactive phosphoprotein 2 antibody|MPM2 reactive phosphoprotein 2 antibody|MPP2 antibody|PIG29 antibody|TGT3 antibody|Transcription factor Trident antibody|Trident antibody|WIN antibody|Winged-helix factor from INS-1 cells antibody|Winged-helix factor from INS1 cells antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- FOXM1 antibody, ASA-B0728, Western blotting
All lanes: Anti FOXM1 (ASA-B0728) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: MCD-7 Whole Cell Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
All lanes: Anti FOXM1 (ASA-B0728) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: COLO320 Whole Cell Lysate at 40ug
Lane 3: SW620 Whole Cell Lysate at 40ug
Lane 4: SKOV Whole Cell Lysate at 40ug
Lane 5: MCD-7 Whole Cell Lysate at 40ug
Predicted bind size: 84KD
Observed bind size: 84KD
Anti- FOXM1 antibody, ASA-B0728, Western blotting
All lanes: Anti FOXM1 (ASA-B0728) at 0.5ug/ml
WB: Recombinant Human FOXM1 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
All lanes: Anti FOXM1 (ASA-B0728) at 0.5ug/ml
WB: Recombinant Human FOXM1 Protein 0.5ng
Predicted bind size: 36KD
Observed bind size: 36KD
