Description
Data Sheet
| Amino acid sequence | MSFRFGQHLI KPSVVFLKTE LSFALVNRKP VVPGHVLVCP LRPVERFHDL RPDEVADLFQ TTQRVGTVVE KHFHGTSLTF SMQDGPEAGQ TVKHVHVHVL PRKAGDFHRN DSIYEELQKH DKEDFPASWR SEEEMAAEAA ALRVYFQLEH HHHHH. |
| Description | FHIT Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids (1-147 a.a.) and having a molecular mass of 17.9 kDa. FHIT protein is fused to an 8 amino acid His tag at C-terminus and is purified by standard chromatography. |
| Expression host | Escherichia Coli. |
| Formulation | FHIT Human solution containing 20mM Tris-HCl pH-8 & 10% glycerol. |
| Protein Background | FHIT enzyme cleaves adenosine 5” PPP 5” A to yield AMP and ADP. FHIT gene includees the regular fragile site FRA3B on chromosome 3. Alterations and deletions of the FHIT gene are highly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach and pancreas. In normal cells, FHIT functions as a tumor suppressor and physically relates with ubiquitin conjugating enzyme 9. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | EC 3.6.1.29, Dinucleosidetriphosphatase, Bis (5”-adenosyl)-triphosphatase , AP3Aase, AP3A hydrolase, Diadenosine 5”,5”””-P1,P3-triphosphate hydrolase, FRA3B. |
