Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| follistatin-like 3(secreted glycoprotein) | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
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| A synthetic peptide corresponding to a sequence in the middle region of human FSTL3(145-159aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
FSTL3 |
Protein |
Follistatin-related protein 3 |
Uniprot ID |
O95633 |
Function |
Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. |
Tissue Specificity |
Expressed in a wide range of tissues. |
Sub-cellular localization |
Isoform 1: Secreted. |
Sequence Similarities |
Contains 2 follistatin-like domains. |
Aliases |
FLRG antibody|follistatin-like 3 antibody|follistatin-like 3 glycoprotein antibody|Follistatin-like protein 3 antibody|Follistatin-related gene protein antibody|Follistatin-related protein 3 antibody|FSRP antibody|Fstl3 antibody|FSTL3_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human Mouse, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-FSTL3 antibody, ASA-B0738, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Human FSTL3, 39.2KD(162aa tag+ L52-P251)
Lane 1: Recombinant Human FSTL3 Protein 10ng
Lane 2: Recombinant Human FSTL3 Protein 5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Human FSTL3, 39.2KD(162aa tag+ L52-P251)
Lane 1: Recombinant Human FSTL3 Protein 10ng
Lane 2: Recombinant Human FSTL3 Protein 5ng
